For Real
There's a character from Persona 5 called Ryuji who said "For real?" a lot.[1][2][3][4]He said it over 20 times in the first stream alone.
At some point, he said "for real" in a more serious and sombre tone than usual. Joe said "The rare serious and sombre "For real" from Ryuji, there".[5]
This meme is represented by the emote 
jph4reel.
References
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“Joseph Anderson Channel Two” video: “Persona 5 - The Pure Positivity Stream (Stream One)” at 02:43:17.
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“Joseph Anderson Channel Two” video: “Persona 5 - The Pure Positivity Stream (Stream One)” at 04:31:30.
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“Joseph Anderson Channel Two” video: “Persona 5 - The Pure Positivity Stream (Stream One)” at 05:03:42.
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“Joseph Anderson Channel Two” video: “Persona 5 - The Pure Positivity Stream (Stream One)” at 05:14:48.
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“Joseph Anderson Channel Two” video: “Persona 5 - Useless (Stream Seven)” at 04:47:42.