2B
2B is the protagonist of NieR: Automata. This was the first weeb game Joe played on stream, so he joked about 2B being his waifu. Examples of this include calling 2B the best waifu, [1] referring to her as the Waifu of the Year, [2] referring to her as a "gateway waifu," [3] and setting her as one of his desktop backgrounds. [4] She is still who people consider to be his waifu today, even after all the other weeb games he's played.
The defunct emote 
jph2Bucket was also a reference to 2B.
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“Joseph Anderson Channel Two” video: “Nier Automata Stream 3” at 00:13:31.
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“Joseph Anderson Channel Two” video: “Xenoblade Chronicles 2 - The Cringe Continues (Stream Three)” at 02:33:36.
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“Falco” video: “"Joseph Anderson" "Don't Starve" with chat [12/24/2017]” at 01:43:45.
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“Falco” video: “"Joseph Anderson Fahrenheit: Indigo Prophecy stream 2 with chat [12/16/2020]” at 00:04:37.