2B

2B is the protagonist of NieR: Automata. This was the first weeb game Joe played on stream, so he joked about 2B being his waifu. She is still who people consider to be his waifu today, even after all the other weeb games he's played.

The defunct emote jph2Bucket was also a reference to 2B. It is no longer enabled on Joe's channel. It depicts 2B holding a bucket because of one part of NieR: Automata where a machine holds a bucket.